Biofertilizers & Plant Vectors

Plant cloning vector

A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA scrap can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a advanced organism, or it may be the plasmid of a bacterium. The vector contains features that allow for the accessible insertion of a DNA scrap into the vector or its removal from the vector, for example through the presence of restriction spots. The vector and the foreign DNA may be treated with a restriction enzyme that cuts the DNA, and DNA fractions therefore generated contain either deaden ends or protuberances known as sticky ends, and vector DNA and foreign DNA with compatible ends can also be joined together by molecular ligation. After a DNA scrap has been reproduced into a cloning vector, it may be further subcloned into another vector designed for more specific use.